Katsura Ise is a writer known for his work on the novel Catcher in the Toilet!, which he and Takuma Yokota adapted into a hit indie manga series that ran from 2007 to 2009. Destroy All Humans. They Can't Be Regenerated is their first collaboration since.

Takuma Yokota is a manga artist who made his professional debut with the one-shot "Tensei Henshin DX Dragon Shaft" ("Reincarnation Transformation DX Dragon Shaft"), which was published in the Winter 2011 issue of Jump Next!. He later had two serializations in Weekly Shonen Jump—Straighten Up! Welcome to Shika High's Competitive Dance Club and Shudan!. Destroy All Humans. They Can't Be Regenerated began serialization in Kadokawa's Shonen Ace magazine in 2018.