This book provides a systematic introduction to biomimetic nanofluidic analytical chemistry, an emerging interdisciplinary field integrating biomimetics, nanofluidics, and analytical chemistry. Organized into four coherent parts?fundamentals, biomimetic design strategies, frontier analytical applications, and future perspectives?it guides readers from nanoscale fluid transport and nanochannel fabrication to bio-inspired smart channel design and selective ion/molecule transport. The book highlights cutting-edge applications such as ultrasensitive sensing, single-molecule and single-cell analysis, salinity-gradient energy generation, CO2 capture, water treatment, point-of-care testing (POCT), and portable analytical devices. With its clear logical structure, emphasis on bio-inspiration and dynamic responsiveness, and broad application scope, the book serves as a comprehensive reference for researchers across chemistry, materials science, biology, medicine, environmental science, and energy.